Homepage of Stephan Preibisch                     

HFSP Fellow in the labs of Robert H. Singer and Eugene Myers at the Albert Einstein College and the MPI-CBG
Visiting Scientist at the HHMI Janelia Farm Research Campus

Globally Optimal Stitching of Tiled 3D
Microscopic Image Acquisitions

Supporting Online Material

Please click on the preview images or the links below to get redirected to the interactive 3D volume browser.

Drosophila larval nervous system
(975 × 1425 × 86, RGB)
Drosophila melanogaster pupae
(1097 × 2345 × 43, 8 Bit Grayscale)
A zone in the dorsal telencephalon of human embryonic tissue
(3570 × 5211 × 70, 8 Bit Grayscale)

Drosophila larval nervous system stained with three dyes, stitched from a grid of 2×3 RGB images. The left side shows the result of the described stitching method. The reconstruction took 1 minute and 20 seconds on a Intel Quad-Core with 2.67 GHz. The right side shows the stitching using the coordinates provided by the microscope stage. One can clearly see misalignments in the overlapping area of the images (see anntotation in the viewer as an example). We thank James W. Truman from Janelia Farm Research Campus for kindly providing the data.

Drosophila melanogaster pupae expressing a GFP reporter under the regulation of the yellow gene, imaged few hours before eclosion using a 4× dry lens on an Optiphot confocal microscope (Nikon). It was stitched from three image stacks arranged in a 1×3 grid. The reconstruction took 42 seconds on a Intel Quad-Core with 2.67 GHz. We thank Nicolas Gompel and Benjamin Prud'homme from IBDML Marseille for kindly providing the data.

A zone in the dorsal telencephalon of human embryonic tissue from week 17 post conception, incubated for 24 hours at 37℃ in DiI. It was imaged using a 63×/1.4 objective on Zeiss LSM 510 equipped with a motorized stage. The final image was created from 24 image stacks arranged in a 4×6 grid. The reconstruction took 22 minutes and 43 seconds on a Intel Quad-Core with 2.67 GHz. We thank Simone Fietz from the lab of Wieland B. Huttner at the MPI-CBG for kindly providing the images.