Homepage of Stephan Preibisch
PhD student in Tomancak Lab at Max Planck Institute of Molecular Cell Biology and Genetics in Dresden
Globally Optimal Stitching of Tiled 3D
Microscopic Image Acquisitions
Supporting Online Material
Please click on the preview images or the links below to get redirected to the interactive 3D volume browser.
- Drosophila larval nervous system
- (975 × 1425 × 86, RGB)
- Drosophila melanogaster pupae
- (1097 × 2345 × 43, 8 Bit Grayscale)
- A zone in the dorsal telencephalon of human embryonic tissue
- (3570 × 5211 × 70, 8 Bit Grayscale)
Drosophila larval nervous system stained with three dyes, stitched from a grid of
2×3 RGB images. The left side shows the result of the described stitching method.
The reconstruction took 1 minute and 20 seconds on a Intel Quad-Core with 2.67 GHz.
The right side shows the stitching using the coordinates provided by the microscope stage.
One can clearly see misalignments in the overlapping area of the images (see anntotation in the viewer as an example).
We thank James
W. Truman from Janelia Farm Research Campus
for kindly providing the data.
Drosophila melanogaster pupae expressing a GFP reporter under the regulation
of the yellow gene, imaged few hours before eclosion using a 4× dry lens
on an Optiphot confocal microscope (Nikon). It was stitched from three image
stacks arranged in a 1×3 grid. The reconstruction took 42 seconds on a
Intel Quad-Core with 2.67 GHz.
We thank Nicolas Gompel
and Benjamin Prud'homme from IBDML Marseille
for kindly providing the data.
A zone in the dorsal telencephalon of human embryonic tissue from week 17
post conception, incubated for 24 hours at 37℃ in DiI. It was
imaged using a 63×/1.4 objective on Zeiss LSM 510 equipped with a
motorized stage. The final image was created from 24 image stacks arranged
in a 4×6 grid. The reconstruction took 22 minutes and 43 seconds on a
Intel Quad-Core with 2.67 GHz.
We thank Simone
Fietz from the lab of Wieland B. Huttner at the
MPI-CBG for kindly providing the images.